Journal: Frontiers in Aging Neuroscience
Article Title: Spinal glial cell derived extra-pituitary prolactin contributes to postoperative pain in females
doi: 10.3389/fnagi.2026.1741102
Figure Lengend Snippet: PRL expression in the spinal cord of sham- and incision-operated female rats and mice. (A, B) PRL/OX-42 (A) and PRL/NeuN co-labeling in the ipsilateral spinal cord (SC) of female rats 1 day after sham. Cyan arrows mark PRL + astrocyte-like cells. NeuN was used as a pan-neuronal marker. Scale bars: 50 μm. (C, C′) PRL labeling in SC 1 day after sham and incision (INC) procedure. Images show dorsal horn regions (laminae I–V; partial ventral laminae). Images were captured with a 10 × objective (A, B, C, C′) . (D) PRL + density in the ipsilateral dorsal horn of SC 1 day after sham and incision (INC) procedure. (E, E′) Immunolabeling of PRL in laminae I–II of the ipsilateral spinal cord (SC) from female mice 1 day after sham surgery (Sham). Yellow arrow indicates OX42 + microglia, and pink arrows indicate PRL − /GFAP + astrocytes. (F, F′) PRL labeling in laminae I–II of the ipsilateral SC from female mice 1 day after incision (INC). Yellow arrow indicates OX42 + microglia, and cyan arrows indicate PRL + /GFAP + astrocytes. Surgical procedures are indicated in row titles. Antibodies and corresponding fluorescence colors are shown in the photomicrographs. Images were captured with a 40 × objective. Scale bar: 25 μm for all panels (E-F′) . (G) Percentages of PRL + cells in NeuN + , Ox-42 + and GFAP + in female mice 1 days after INC.
Article Snippet: The following antibodies were used in the study: PRL protein in rats was detected with rabbit anti-PRL (Agilent-DAKO, Santa Clara, CA; cat: A0569; 1:100) ( ); PRL in mice was detected with rabbit anti-PRL (Bioss, Boston, MA; cat: BS-23763R; 1:200); pSTAT5 in rats was detected with rabbit anti-pSTAT5 (Tyr694, Cell Signaling Technology, Beverly, MA; cat: 9314S; 1:200) ( ); rat DRG and spinal cord neurons were detected with mouse monoclonal anti-NeuN antibodies (Millipore-Sigma, St. Louis, MO; catalog MAB377; 1:100); mouse spinal astrocytes and Schwann cells in hind paw were labeled with chicken anti-GFAP (Neuromics, Edina, MN; catalog CH22102; 1:100); and rat and mouse monocytes/macrophages/dendritic cells/microglia were labeled with mouse monoclonal antibody OX-42 (CD11b/c; Bio-Rad, Hercules, CA; catalog MCA275GA; 1:50) ( ).
Techniques: Expressing, Labeling, Marker, Immunolabeling, Fluorescence
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